MCM7 Rabbit Polyclonal Antibody
cat.: ER1902-60
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size 81/60/45 kDa.
Isotype: IgG
Immunogen: Recombinant protein corresponding to C terminal Human MCM7.
Positive control: MCF-7 cell lysate, K562 cell lysate, mouse colon tissue lysate, MCF-7, MG-63, SHSY5Y, human lung cancer tissue, human colon tissue, mouse skin tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P33993 Human | Q61881 Mouse | Q6AYN8 Rat
Alternative names: CDABP0042 CDC 47 CDC47 CDC47 homolog Cdc47, S. cerevisiae, homolog of DNA replication licensing factor MCM7 Homolog of S. cerevisiae Cdc47 MCM 2 MCM 7 MCM2 MCM2, formerly Mcm7 MCM7 minichromosome maintenance deficient 7 MCM7_HUMAN Minichromosome Maintainence 7 Minichromosome maintainence, S. cerevisiae, homolog of Minichromosome maintenance complex component 7 Minichromosome maintenance deficient 7 Minichromosome maintenance protein 7 P1.1 MCM3 P1.1-MCM3 P1CDC47 P85MCM PNAS 146 PNAS146 PPP1R104 Protein phosphatase 1, regulatory subunit 104
Images
ER1902-60_1.jpg Fig1: Western blot analysis of MCM7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: K562 cell lysate
Lane 3: Mouse colon tissue lysate
ER1902-60_2.jpg Fig2: ICC staining of MCM7 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-60, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1902-60_3.jpg Fig3: ICC staining of MCM7 in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-60, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1902-60_4.jpg Fig4: ICC staining of MCM7 in SHSY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-60, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1902-60_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MCM7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-60_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MCM7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-60, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-60_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-MCM7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-60, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-60_8.jpg Fig8: Flow cytometric analysis of MCM7 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-60, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; green).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.