ATP8A1 Rabbit Polyclonal Antibody
cat.: ER1902-61
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 131 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ATP8A1 aa 410-637 / 1,164. |
| Positive control: | MCF-7 cell lysate, rat brain tissue lysate, MCF-7. |
| Subcellular location: | Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9Y2Q0 Human | A0A8I5ZXX6 Rat |
| Alternative names: | Phospholipid-transporting ATPase IA ATPase class I type 8A member 1 Chromaffin granule ATPase II P4-ATPase flippase complex alpha subunit ATP8A1 ATP8A1 |
Images
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Fig1:
Western blot analysis of ATP8A1 on different lysates with Rabbit anti-ATP8A1 antibody (ER1902-61) at 1/1,000 dilution. Lane 1: Mouse brain Lane 2: Rat brain Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1902-61, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 131 kDa Observed band size: 120 kDa |
|
Fig2: Flow cytometric analysis of ATP8A1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-61, 1/50) (yellow). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; purple). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.