DNMT3A Rabbit Polyclonal Antibody
cat.: ER1902-62
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size 102 kDa.
Isotype: IgG
Immunogen: Recombinant protein within human DNMT3A aa 1-300.
Positive control: Hela cell lysates, rat large intestine tissue, human tonsil tissue, human breast carcinoma tissue, human kidney tissue, human fetal skeletal muscle tissue, mouse brain tissue, SH-SY5Y.
Subcellular location: Nucleus, cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9Y6K1 Human | O88508 Mouse | Q1LZ53 Rat
Alternative names: DNA (cytosine 5) methyltransferase 3 alpha DNA (cytosine 5) methyltransferase 3A DNA (cytosine-5)-methyltransferase 3A DNA cytosine methyltransferase 3A2 DNA methyltransferase 3 alpha DNA methyltransferase 3a DNA methyltransferase HsaIIIA DNA MTase HsaIIIA DNM3A_HUMAN DNMT 3a DNMT Dnmt3a DNMT3A2 M.HsaIIIA MCMT OTTHUMP00000201149 TBRS
Images
ER1902-62_1.jpg Fig1: Western blot analysis of DNMT3A on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ER1902-62_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-DNMT3A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-62, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-62_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DNMT3A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-62, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-62_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DNMT3A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-62, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-62_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DNMT3A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-62, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-62_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-DNMT3A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-62, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-62_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DNMT3A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-62, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-62_8.jpg Fig8: Flow cytometric analysis of DNMT3A was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-62, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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