FEN1 Rabbit Polyclonal Antibody
cat.: ER1902-64
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human FEN1 aa 170-380 / 380.
Positive control: K-562 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, MCF-7, SHSY5Y, human tonsil tissue, human lung cancer tissue, human colon tissue, mouse testis tissue, MG-63.
Subcellular location: Nucleus, nucleolus, nucleoplasm; Mitochondrion.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P39748 Human | P39749 Mouse | Q5XIP6 Rat
Alternative names: DNase IV FEN-1 FEN1 FEN1_HUMAN Flap endonuclease 1 Flap structure specific endonuclease 1 Flap structure-specific endonuclease 1 hFEN-1 hFEN1 Maturation factor 1 MF1 Rad2
Images
ER1902-64_1.jpg Fig1: Western blot analysis of FEN1 on different lysates with Rabbit anti-FEN1 antibody (ER1902-64) at 1/1,000 dilution.

Lane 1: K-562 cell lysate (10 µg/Lane)
Lane 2: NIH/3T3 cell lysate (10 µg/Lane)
Lane 3: PC-12 cell lysate (10 µg/Lane)
Lane 4: Mouse testis tissue lysate (20 µg/Lane)

Predicted band size: 43 kDa
Observed band size: 45 kDa

Exposure time: 2 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-64) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER1902-64_2.jpg Fig2: ICC staining of FEN1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-64, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1902-64_3.jpg Fig3: ICC staining of FEN1 in SHSY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-64, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1902-64_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FEN1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-64, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-64_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-FEN1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-64, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-64_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-FEN1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-64, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-64_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-FEN1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-64, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-64_8.jpg Fig8: Flow cytometric analysis of FEN1 was done on MG-63 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-64, 1/100) (yellow). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; purple).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.