RIP3 Rabbit Polyclonal Antibody
cat.: ER1902-67
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RIP3 aa 380-420. |
| Positive control: | Human lung carcinoma tissue lysate, human placenta tissue lysate, rat kidney tissue, SW620, HT-29 cell lysates. |
| Subcellular location: | Cytoplasm, cytosol, Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9Y572 Human | Q9QZL0 Mouse | Q9Z2P5 Rat |
| Alternative names: | Receptor interacting protein 3 Receptor interacting serine threonine kinase 3 Receptor interacting serine/threonine protein kinase 3 Receptor-interacting protein 3 Receptor-interacting serine/threonine-protein kinase 3 RIP 3 RIP like protein kinase 3 RIP-3 RIP-like protein kinase 3 RIPK 3 RIPK3 RIPK3_HUMAN |
Images
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Fig1:
Western blot analysis of RIP3 on HT-29 cell lysates with Rabbit anti-RIP3 antibody (ER1902-67) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-67) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of RIP3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human lung carcinoma tissue lysate Lane 2: human placenta tissue lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-RIP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of RIP3 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-67, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.