TTF-1 Rabbit Polyclonal Antibody
cat.: ER1902-68
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size 42/39 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within Human TTF-1 aa 300-349 / 371.
Positive control: Mouse lung tissue lysate, rat lung tissue lysate, 293, Hela.
Subcellular location: Nucleus
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: P43699 Human | P50220 Mouse | P23441 Rat
Alternative names: AV026640 BCH Benign chorea BHC Homeobox protein NK 2 homolog A Homeobox protein NK-2 homolog A Homeobox protein Nkx 2.1 Homeobox protein Nkx-2.1 Homeobox protein Nkx2.1 NK 2 NK 2 homolog A NK2 NK2 homeobox 1 NK2, drosophila, homolog of, A NK2.1, mouse, homolog of Nkx 2 1 NKX 2.1 NKX 2A NKX2 1 Nkx2-1 NKX2.1 NKX21_HUMAN NKX2A T EBP T/EBP TEBP Thyroid nuclear factor 1 Thyroid nuclear factor Thyroid specific enhancer binding protein Thyroid transcription factor 1 Tin man Tinman TITF 1 TITF1 TTF 1 TTF-1 TTF1
Images
ER1902-68_1.jpg Fig1: Western blot analysis of TTF-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse lung tissue lysate
Lane 2: Rat lung tissue lysate
ER1902-68_2.png Fig2: Western blot analysis of TTF-1 on different lysates with Rabbit anti-TTF-1 antibody (ER1902-68) at 1/500 dilution.

Lane 1: 293 cell lysate
Lane 2: Hela cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 42/39 kDa
Observed band size: 42 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-68) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.