TTF-1 Rabbit Polyclonal Antibody
cat.: ER1902-68
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size 42/39 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TTF-1 aa 300-349 / 371. |
| Positive control: | Mouse lung tissue lysate, rat lung tissue lysate, 293, Hela. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P43699 Human | P50220 Mouse | P23441 Rat |
| Alternative names: | AV026640 BCH Benign chorea BHC Homeobox protein NK 2 homolog A Homeobox protein NK-2 homolog A Homeobox protein Nkx 2.1 Homeobox protein Nkx-2.1 Homeobox protein Nkx2.1 NK 2 NK 2 homolog A NK2 NK2 homeobox 1 NK2, drosophila, homolog of, A NK2.1, mouse, homolog of Nkx 2 1 NKX 2.1 NKX 2A NKX2 1 Nkx2-1 NKX2.1 NKX21_HUMAN NKX2A T EBP T/EBP TEBP Thyroid nuclear factor 1 Thyroid nuclear factor Thyroid specific enhancer binding protein Thyroid transcription factor 1 Tin man Tinman TITF 1 TITF1 TTF 1 TTF-1 TTF1 |
Images
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Fig1:
Western blot analysis of TTF-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse lung tissue lysate Lane 2: Rat lung tissue lysate |
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Fig2:
Western blot analysis of TTF-1 on different lysates with Rabbit anti-TTF-1 antibody (ER1902-68) at 1/500 dilution. Lane 1: 293 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 42/39 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-68) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.