TRF2 Rabbit Polyclonal Antibody
cat.: ER1902-70
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 60 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human TRF2 aa 124-301 / 542.
Positive control: A549, LOVO, Siha, rat uterus tissue, human tonsil tissue, mouse testis tissue.
Subcellular location: Nucleus, Chromosome, telomere.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q15554 Human | O35144 Mouse | D3ZJF7 Rat
Alternative names: Telomeric DNA binding protein Telomeric DNA-binding protein Telomeric repeat binding factor 2 Telomeric repeat binding protein 2 Telomeric repeat-binding factor 2 TERF 2 Terf2 TERF2_HUMAN TRBF 2 TRBF2 TRF 2 TRF2 TTAGGG repeat binding factor 2 TTAGGG repeat-binding factor 2
Images
ER1902-70_1.jpg Fig1: Western blot analysis of TRF2 on A549 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-70_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-TRF2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1902-70) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1902-70_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TRF2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1902-70) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1902-70_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TRF2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1902-70) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1902-70_5.jpg Fig5: Flow cytometric analysis of TRF2 was done on Siha cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-70, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.