Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size 49 kDa. |
Isotype: | IgG |
Immunogen: | Recombinant protein corresponding to N terminal Human MSR1. |
Positive control: | Hela cell lysates, SHSY5Y. |
Subcellular location: | Membrane |
Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
Uniprot #: | SwissProt: P21757 Human |
Alternative names: | CD204 CD204 antigen CD24 Macrophage acetylated LDL receptor I and II Macrophage scavenger receptor 1 Macrophage scavenger receptor type III Macrophage scavenger receptor types I and II Msr 1 MSR1 MSRE_HUMAN phSR1 phSR2 SCARA 1 SCARA1 Scavenger receptor class A member 1 Scavenger receptor class A, member 1 Scavenger receptor type A Scvr SR A SRA |
Fig1: Western blot analysis of MSR1 on hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. | |
Fig2: Flow cytometric analysis of MSR1 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-71, 1/100) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow). |