CDK5RAP3 Rabbit Polyclonal Antibody
cat.: ER1902-72
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size 57 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CDK5RAP3 aa 296-506 / 506. |
| Positive control: | MCF-7 cell lysates, LOVO, PC-3M, SHSY5Y. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q96JB5 Human |
| Alternative names: | C53 CDK5 activator binding protein C53 CDK5 activator-binding protein C53 CDK5 regulatory subunit associated protein 3 CDK5 regulatory subunit-associated protein 3 Cdk5rap3 CK5P3_HUMAN HSF 27 HSF27 IC53 Ischemic heart CDK5 activator binding protein C53 LXXLL/leucine zipper containing ARFbinding protein LZAP MST016 OK/SW cl.114 PP1553 Protein HSF-27 |
Images
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Fig1: Western blot analysis of CDK5RAP3 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of CDK5RAP3 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-72, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of CDK5RAP3 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-72, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of CDK5RAP3 in SHSY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-72, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: Flow cytometric analysis of CDK5RAP3 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-72, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.