Anti-USP29 antibody
cat.: ER1902-76
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 104 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human USP29 aa 665-942.
Positive control: THP-1 cell lysate, rat testis tissue lysate, 293.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  FC

1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: Q9HBJ7 Human | D4AEI6 Rat
Alternative names: Deubiquitinating enzyme 29 antibody HOM TES 84/86 antibody MGC163266 antibody MGC163270 antibody Ubiquitin carboxyl terminal hydrolase 29 antibody Ubiquitin carboxyl-terminal hydrolase 29 antibody Ubiquitin specific peptidase 29 antibody Ubiquitin specific processing protease 29 antibody Ubiquitin specific processing protease antibody Ubiquitin thiolesterase 29 antibody Ubiquitin-specific-processing protease 29 antibody UBP29_HUMAN antibody USP 29 antibody Usp29 antibody
Images
ER1902-76_1.jpg Fig1: Western blot analysis of USP29 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: THP-1 cell lysate
Lane 2: Rat testis tissue lysate
ER1902-76_2.jpg Fig2: Flow cytometric analysis of USP29 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-76, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.