USP29 Rabbit Polyclonal Antibody
cat.: ER1902-76
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 104 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human USP29 aa 665-922 / 922. |
| Positive control: | Rat testis tissue lysate, mouse testis tissue lysate, THP-1 cell lysate, 293. |
| Subcellular location: | Cytoplasm, perinuclear region. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9HBJ7 Human | Q9ES63 Mouse | D4AEI6 Rat |
| Alternative names: | Deubiquitinating enzyme 29 HOM TES 84/86 MGC163266 MGC163270 Ubiquitin carboxyl terminal hydrolase 29 Ubiquitin carboxyl-terminal hydrolase 29 Ubiquitin specific peptidase 29 Ubiquitin specific processing protease 29 Ubiquitin specific processing protease Ubiquitin thiolesterase 29 Ubiquitin-specific-processing protease 29 UBP29_HUMAN USP 29 Usp29 |
Images
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Fig1:
Western blot analysis of USP29 on different lysates with Rabbit anti-USP29 antibody (ER1902-76) at 1/500 dilution. Lane 1: Rat testis tissue lysate Lane 2: Mouse testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 104 kDa Observed band size: 104 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-76) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of USP29 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate Lane 2: Rat testis tissue lysate |
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Fig3: Flow cytometric analysis of USP29 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-76, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.