IFITM1 Rabbit Polyclonal Antibody
cat.: ER1902-77
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 14 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human IFITM1 aa 1-50 / 125.
Positive control: K562 cell lysate, SHG-44 cell lysate, human tonsil tissue, human spleen tissue, K562.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P13164 Human
Alternative names: 9-27 CD 225 CD 225 antigen CD225 CD225 antigen Dispanin subfamily A member 2a DSPA2a IFI 17 IFI17 IFITM1 IFM1_HUMAN Interferon induced protein 17 interferon induced transmembrane protein 1 (9-27) Interferon induced transmembrane protein 1 Interferon inducible protein 9-27 Interferon-induced protein 17 Interferon-induced transmembrane protein 1 Interferon-inducible protein 9-27 Leu 13 Leu 13 antigen Leu-13 antigen LEU13
Images
ER1902-77_1.jpg Fig1: Western blot analysis of IFITM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: SHG-44 cell lysate
ER1902-77_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IFITM1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-77, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-77_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IFITM1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-77, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-77_4.jpg Fig4: Flow cytometric analysis of IFITM1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-77, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.