Anti-Rab11-FIP3 antibody
cat.: ER1902-80
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: Predicted band size 82/119 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within mouse FIP3 aa 550-590.
Positive control: MCF-7 cell lysates, mouse liver tissue, mouse kindey tissue, MCF-7.
Subcellular location: Recycling endosome membrane, centrosome, Cleavage furrow, Midbody.
Recommended Dilutions:
  WB
  IHC
  FC

1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O75154 Human | Q8CHD8 Mouse
Alternative names: EF hands containing Rab interacting protein Eferin Rab11 family interacting protein 3 RAB11FIP3 RFIP3
Images
ER1902-80_1.jpg Fig1: Western blot analysis of Rab11-FIP3 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-80, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-80_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Rab11-FIP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-80, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-80_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse kindey tissue using anti-Rab11-FIP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-80, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-80_4.jpg Fig4: Flow cytometric analysis of Rab11-FIP3 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-80, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.