RAB11FIP3 Rabbit Polyclonal Antibody
cat.: ER1902-80
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 119 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse RAB11FIP3 aa 543-592 / 1,047. |
| Positive control: | U-87 MG cell lysate, AGS cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, human liver tissue, human kidney tissue. |
| Subcellular location: | Endosome membrane, Recycling endosome membrane, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cleavage furrow, Midbody, Golgi apparatus membrane, Golgi apparatus, trans-Golgi network membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:5,000 |
| Uniprot #: | SwissProt: O75154 Human | Q8CHD8 Mouse Entrez Gene: 303002 Rat |
| Alternative names: | EF hands containing Rab interacting protein Eferin Rab11 family interacting protein 3 RAB11FIP3 RFIP3 |
Images
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Fig1:
Western blot analysis of RAB11FIP3 on different lysates with Rabbit anti-RAB11FIP3 antibody (ER1902-80) at 1/5,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: AGS cell lysate Lane 3: Mouse liver tissue lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat liver tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 119 kDa Observed band size: 119 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-80) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-RAB11FIP3 antibody (ER1902-80) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-80) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-RAB11FIP3 antibody (ER1902-80) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-80) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.