TRPM4 Rabbit Polyclonal Antibody
cat.: ER1902-81
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: Predicted band size 134 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within Human TRPM4 aa 1-50 / 1,214.
Positive control: SK-Br-3 cell lysates, rat heart tissue, mouse small intestine tissue, MCF-7.
Subcellular location: Cell membrane, endoplasmic reticulum, golgi apparatus.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q8TD43 Human | Q7TN37 Mouse | Q9ESQ5 Rat
Alternative names: 1110030C19Rik AW047689 Calcium-activated non-selective cation channel 1 FLJ20041 hTRPM4 Long transient receptor potential channel 4 LTrpC-4 LTrpC4 Melastatin 4 Melastatin like 2 protein Melastatin-4 Melastatin-like 2 Mls2s PFHB1B Transient receptor potential cation channel subfamily M member 4 Transient receptor potential cation channel, subfamily M, member 4 Trpm4 TRPM4_HUMAN TRPM4B
Images
ER1902-81_1.jpg Fig1: Western blot analysis of TRPM4 on SK-Br-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-81_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-TRPM4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-81_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-TRPM4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-81_4.jpg Fig4: Flow cytometric analysis of TRPM4 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-81, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.