LIM1 Rabbit Polyclonal Antibody
cat.: ER1902-83
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IHC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 45 kDa
Isotype: IgG
Immunogen: Synthetic peptide within zebrafish LIM1 aa 144-193 / 405.
Positive control: A431 cell lysate, zebrafish tissue lysate, rat brian tissue, mouse brian tissue, MCF-7.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P48742 Human | P63006 Mouse | P63007 Rat | Q90476 Zebrafish
Alternative names: hLim-1 Homeo box protein Lim 1 Homeo box protein Lim1 Homeobox protein Lim 1 Homeobox protein Lim-1 Homeobox protein Lim1 LHX 1 LHX1 LHX1_HUMAN LIM 1 LIM homeo box 1 LIM homeo box protein 1 LIM homeobox 1 LIM homeobox protein 1 LIM-1 LIM/homeobox protein Lhx 1 LIM/homeobox protein Lhx1 MGC126723 MGC138141
Images
ER1902-83_1.jpg Fig1: Western blot analysis of LIM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-83, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: Zebrafish tissue lysate
ER1902-83_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brian tissue using anti-LIM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-83_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brian tissue using anti-LIM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-83_4.jpg Fig4: Flow cytometric analysis of LIM1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-83, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.