SLFN12 Rabbit Polyclonal Antibody
cat.: ER1902-84
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 67 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human SLFN12 aa 529-578 / 578.
Positive control: U937 cell lysate, HL-60 cell lysate, human lung tissue, human prostate tissue, human small intestine tissue, human pancreas tissue, 293.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q8IYM2 Human
Alternative names: FLJ10260 Schlafen family member 12 schlafen family member12 SLFN12 SLFN3 SLN12_HUMAN
Images
ER1902-84_1.jpg Fig1: Western blot analysis of SLFN12 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-84, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: U937 cell lysate
Lane 2: HL-60 cell lysate
ER1902-84_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-SLFN12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-84, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-84_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-SLFN12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-84, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-84_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-SLFN12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-84, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-84_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-SLFN12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-84, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-84_6.jpg Fig6: Flow cytometric analysis of SLFN12 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-84, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.