CPEB1 Rabbit Polyclonal Antibody
cat.: ER1902-89
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 63 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human CPEB1 aa 70-110.
Positive control: Rat skin tissue lysates, rat brain tissue, human liver cancer tissue, human colon cancer tissue, mouse brain tissue, K562.
Subcellular location: Nucleus, Cytoplasm, P-body, Cytoplasmic granule, synapse, Membrane, postsynaptic density, dendrite.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:1,000
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: Q9BZB8 Human | P70166 Mouse | P0C279 Rat
Alternative names: CEBP CPE binding protein 1 CPE BP1 CPE-binding protein 1 CPE-BP1 CPEB 1 CPEB CPEB-1 CPEB1 CPEB1 protein CPEB1_HUMAN Cytoplasmic polyadenylation binding protein 1 Cytoplasmic polyadenylation element binding protein Cytoplasmic polyadenylation element binding protein 1 Cytoplasmic polyadenylation element-binding protein 1 FLJ13203 h CEBP h-CEBP hCPEB 1 hCPEB-1 MGC34136 MGC60106 mKIAA0940 MKIAA0940 protein
Images
ER1902-89_1.jpg Fig1: Western blot analysis of CPEB1 on rat skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-89_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CPEB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-89, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-89_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CPEB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-89, 1100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-89_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CPEB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-89, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-89_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CPEB1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-89, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-89_6.jpg Fig6: Flow cytometric analysis of CPEB1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-89, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.