ERN1 Rabbit Polyclonal Antibody
cat.: ER1902-90
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Peptide affinity purified.
Molecular weight: Predicted band size 110 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal Human ERN1.
Positive control: C2C12 cell lysate, HepG2 cell lysate, rat uterus tissue, human appendix tissue.
Subcellular location: Endoplasmic reticulum, Membrane.
Recommended Dilutions:
  WB
  IHC-P

1:500
1:50-1:200
Uniprot #: SwissProt: O75460 Human
Alternative names: Endoplasmic reticulum (ER) to nucleus signalling 1 Endoplasmic reticulum to nucleus signaling 1 Endoplasmic reticulum-to-nucleus signaling 1 Endoribonuclease ER to nucleus signaling 1 ERN 1 Ern1 ERN1_HUMAN hIRE 1p hIRE1p Inositol requiring 1 Inositol requiring 1, S. cerevisiae, homolog of Inositol requiring enzyme 1, S. cerevisiae, homolog of Inositol requiring protein 1 inositol-requiring enzyme 1 Inositol-requiring protein 1 IRE 1 IRE 1a IRE 1P Ire1 alpha Ire1-alpha IRE1a Ire1alpha IRE1P MGC163277 MGC163279 Protein kinase/endoribonuclease RGD1559716 Serine/threonine protein kinase/endoribonuclease IRE1
Images
ER1902-90_1.jpg Fig1: Western blot analysis of ERN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: C2C12 cell lysate
Lane 2: HepG2 cell lysate
ER1902-90_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-ERN1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-90, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-90_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-ERN1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-90, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.