IRE1 Rabbit Polyclonal Antibody
cat.: ER1902-90
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 0.2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size 110 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal Human ERN1. |
| Positive control: | HEK-293 cell lysate, Neuro-2a cell lysate, C6 cell lysate, mouse small intestine tissue, rat small intestine tissue. |
| Subcellular location: | Endoplasmic reticulum, Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:100 |
| Uniprot #: | SwissProt: O75460 Human |
| Alternative names: | Endoplasmic reticulum (ER) to nucleus signalling 1 Endoplasmic reticulum to nucleus signaling 1 Endoplasmic reticulum-to-nucleus signaling 1 Endoribonuclease ER to nucleus signaling 1 ERN 1 Ern1 ERN1_HUMAN hIRE 1p hIRE1p Inositol requiring 1 Inositol requiring 1, S. cerevisiae, homolog of Inositol requiring enzyme 1, S. cerevisiae, homolog of Inositol requiring protein 1 inositol-requiring enzyme 1 Inositol-requiring protein 1 IRE 1 IRE 1a IRE 1P Ire1 alpha Ire1-alpha IRE1a Ire1alpha IRE1P MGC163277 MGC163279 Protein kinase/endoribonuclease RGD1559716 Serine/threonine protein kinase/endoribonuclease IRE1 |
Images
|
Fig1:
Western blot analysis of IRE1 on different lysates with Rabbit anti-IRE1 antibody (ER1902-90) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-90) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-IRE1 antibody (ER1902-90) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-90) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-IRE1 antibody (ER1902-90) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-90) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.