D14 Rabbit Polyclonal Antibody
cat.: ER1902-91
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Rice
Applications: WB, IHC-P, IF
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Peptide affinity purified.
Molecular weight: Predicted band size 33 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal Rice D14.
Positive control: Rice.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF

1:500-1:1000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q10QA5
Alternative names: Strigolactone esterase D1 Protein DWARF 14 , rotein DWARF 88, Protein HIGH-TILLERING DWARF 2 D14
Images
ER1902-91_1.jpg Fig1: Western blot analysis of D14 on rice tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-91_2.jpg Fig2: Immunofluorescence staining of paraffin- embedded rice tissue using anti-D14 rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH 6.0) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody () at 1/100 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
ER1902-91_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rice tissue using anti-D14 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody () at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.