MFF Rabbit Polyclonal Antibody
cat.: ER1902-93
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 1% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MFF aa 1-342. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, 293T cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Mitochondrion outer membrane, Peroxisome, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q9GZY8 Human | Q6PCP5 Mouse | Q4KM98 Rat |
| Alternative names: | C2orf33 Chromosome 2 open reading frame 33 DKFZp666J168 GL004 Mff MFF_HUMAN MGC110913 Mitochondrial fission factor OTTHUMP00000164235 |
Images
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Fig1:
Western blot analysis of MFF on different lysates with Rabbit anti-MFF antibody (ER1902-93) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 30 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-93) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.