Anti-MFF antibody
cat.: ER1902-93
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 38 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human MFF aa 160-200.
Positive control: Hela cell lysate, HepG2 cell lysate, rat testis tissue, mouse testis tissue, K562.
Subcellular location: Mitochondrion outer membrane, Peroxisome, synaptic vesicle.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: Q9GZY8 Human | Q6PCP5 Mouse | Q4KM98 Rat
Alternative names: C2orf33 Chromosome 2 open reading frame 33 DKFZp666J168 GL004 Mff MFF_HUMAN MGC110913 Mitochondrial fission factor OTTHUMP00000164235
Images
ER1902-93_1.jpg Fig1: Western blot analysis of MFF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
ER1902-93_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-MFF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-93, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-93_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MFF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-93, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-93_4.jpg Fig4: Flow cytometric analysis of MFF was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-93, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.