CHRNB2 Rabbit Polyclonal Antibody
cat.: ER1902-95
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 57 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within rat CHRNB2 aa 50-150.
Positive control: Jurkat cell lysates, A549, SH-SY5Y, rat spinal cord tissue, mouse brain tissue.
Subcellular location: Postsynaptic cell membrane, cell membrane.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50-1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P17787 Human | Q9ERK7 Mouse | P12390 Rat
Alternative names: Acetylcholine receptor beta 2 neural ACHB2_HUMAN ACHN AChR Acrb 2 Acrb2 b2 nAchR Cholinergic receptor nicotinic beta 2 Cholinergic receptor nicotinic beta polypeptide 2 Cholinergic receptor nicotinic beta polypeptide 2 neuronal cholinergic receptor, nicotinic, beta 2 (neuronal) Chrnb2 EFNL 3 EFNL3 nAChRB2 Neuronal acetylcholine receptor protein beta 2 chain precursor Neuronal acetylcholine receptor protein subunit beta 2 Neuronal acetylcholine receptor subunit beta-2 Neuronal nicotinic acetylcholine receptor beta 2
Images
ER1902-95_1.jpg Fig1: Western blot analysis of CHRNB2 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-95, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-95_2.jpg Fig2: ICC staining of CHRNB2 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-95, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1902-95_3.jpg Fig3: ICC staining of CHRNB2 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-95, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1902-95_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-CHRNB2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-95, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-95_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CHRNB2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-95, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-95_6.jpg Fig6: Flow cytometric analysis of CHRNB2 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-95, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.