PICK1 Rabbit Polyclonal Antibody
cat.: ER1902-97
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size 47/40 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within rat PICK1 aa 280-330.
Positive control: SH-SY5Y cell lysate, 293 cell lysate, F9, MCF-7, rat brain tissue, human colon tissue, mouse brain tissue, SH-SY5Y.
Subcellular location: Cytoskeleton, perinuclear region, membrane, postsynaptic density, synaptosome.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:1,000
1:50-1:100
1:50-1:200
1:100-1:500
Uniprot #: SwissProt: Q9NRD5 Human | Q62083 Mouse | Q9EP80 Rat
Alternative names: dJ1039K5 MGC15204 OTTHUMP00000028509 PICK 1 Pick1 PICK1_HUMAN PRKCA binding protein PRKCA-binding protein PRKCABP Protein interacting with C kinase 1 Protein interacting with PRKCA Protein interacting with PRKCA 1 Protein kinase C alpha binding protein Protein kinase C-alpha-binding protein Protein that interacts with C kinase 1
Images
ER1902-97_1.jpg Fig1: Western blot analysis of PICK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-97, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SH-SY5Y cell lysate
Lane 2: 293 cell lysate
ER1902-97_2.jpg Fig2: ICC staining of PICK1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-97, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1902-97_3.jpg Fig3: ICC staining of PICK1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-97, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1902-97_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PICK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-97, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-97_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PICK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-97, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-97_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PICK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-97, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-97_7.jpg Fig7: Flow cytometric analysis of PICK1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-97, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.