MARCH9 Rabbit Polyclonal Antibody
cat.: ER1903-04
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MARCH9 aa 21-70. |
| Positive control: | MCF7 cell lysate, A549 cell lysate. |
| Subcellular location: | Golgi apparatus membrane, Lysosome membrane. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q86YJ5 Human |
| Alternative names: | BC019560 E3 ubiquitin protein ligase MARCH9 FLJ36578 MARCH IX Membrane associated RING CH protein IX membrane associated ring finger (C3HC4) 9 Membrane associated RING finger protein 9 RING finger protein 179 RNF179 |
Images
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Fig1:
Western blot analysis of MARCH9 on different lysates with Rabbit anti-MARCH9 antibody (ER1903-04) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 38 kDa Observed band size: 45 kDa Exposure time: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1903-04) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.