Aurora C Rabbit Polyclonal Antibody
cat.: ER1904-04
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Aurora C |
| Positive control: | HCT 116 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, Huh7 cell lysate, HeLa cell lysate, PC-12 cell lysate, Mouse testis tissue lysate. |
| Subcellular location: | Nucleus, Chromosome, centromere, Cytoplasm, cytoskeleton, spindle. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q9UQB9 Human | O88445 Mouse Entrez Gene: 292554 Rat |
| Alternative names: | AURKC AIE 2 Aie1 AIE2 AIK 3 AIK3 ARK 3 ARK-3 ARK3 Aur C AurC Aurkc AURKC_HUMAN aurora 3 Aurora C Aurora kinase C aurora related kinase 3 Aurora-related kinase 3 Aurora/Ipl1 related kinase 3 Aurora/Ipl1-related kinase 3 Aurora/Ipl1/Eg2 protein 2 EC 2.7.11.1 Serine threonine protein kinase 13 serine/threonine kinase 13 (aurora/IPL1 like) serine/threonine protein kinase aurora C Serine/threonine-protein kinase 13 Serine/threonine-protein kinase aurora-C SPGF5 STK13 |
Images
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Fig1:
Western blot analysis of Aurora C on different lysates with Rabbit anti-Aurora C antibody (ER1904-04) at 1/5,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: Huh7 cell lysate Lane 5: HeLa cell lysate Lane 6: PC-12 cell lysate Lane 7: Mouse testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 32 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1904-04) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.