Calcitonin Rabbit Polyclonal Antibody
cat.: ER1905-32
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 3.5/13 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Calcitonin 85-116/141 |
| Positive control: | Mouse brain tissue, Cerebrum (Mouse) Lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:100-500 1:100-500 |
| Uniprot #: | SwissProt: P01258 Human |
| Alternative names: | Alpha CGRP alpha type CGRP CALC 1 CALC A CALC1 calcitonin 1 calcitonin gene related peptide 1 calcitonin gene related peptide I calcitonin related polypeptide alpha CGRP 1 CGRP CGRP I CGRP1 CT katacalcin KC |
Images
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Fig1:
Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Calcitonin (bs-18210R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 3.5/13 kD Observed band size: 17 kD |
|
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calcitonin) Polyclonal Antibody, Unconjugated (bs-18210R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.