CAMKK2 Rabbit Polyclonal Antibody
cat.: ER1905-35
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, IF-Tissue, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 55 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human CAMKK2 501-588/588 |
| Positive control: | Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung. |
| Subcellular location: | Nucleus. Cytoplasm. Cell projection. Note=Predominantly nuclear in unstimulated cells. Found in the cytoplasm and neurites after forskolin induction. |
| Recommended Dilutions:
WB IHC-P IF-tissue FC |
1:500-2000 1:100-500 1:100-500 1ug/Test |
| Uniprot #: | SwissProt: Q96RR4 Human |
| Alternative names: | Calcium/calmodulin dependent protein kinase beta Calcium/calmodulin dependent protein kinase kinase 2 Calcium/calmodulin dependent protein kinase kinase 2 beta Calcium/calmodulin dependent protein kinase kinase beta Calcium/calmodulin-dependent protein kinase kinase 2 Calcium/calmodulin-dependent protein kinase kinase beta CaM kinase kinase beta CaM KK beta CaM-kinase kinase 2 CaM-kinase kinase beta CaM-KK 2 CaM-KK beta CaMKK 2 CAMKK CaMKK beta CAMKK beta protein Camkk2 CAMKKB KKCC2_HUMAN |
Images
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Fig1:
Sample: Lane 1: Human Jurkat cell lysates Lane 2: Human MOLT4 cell lysates Lane 3: Human HepG2 cell lysates Primary: Anti-CAMKK2 (bs-6253R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 70 kDa |
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Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (CAMKK2) Polyclonal Antibody, Unconjugated (bs-6253R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. |
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Fig3:
Blank control (Black line): Molt4 (Black). Primary Antibody (green line):Rabbit Anti-CAMKK2 antibody (bs-6253R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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Fig4: Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CAMKK2 Antibody(bs-6253R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.