Cathepsin B Rabbit Polyclonal Antibody
cat.: ER1905-48
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 23/28/37 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Cathepsin B heavy chain 251-339/339 |
| Positive control: | Human esophageal carcinoma, Mouse Spleen Lysate. |
| Subcellular location: | Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Recommended Dilutions:
WB IHC-P IF-tissue FC |
1:500-2000 1:100-500 1:100-500 1µg/Test |
| Uniprot #: | SwissProt: P10605 Mouse |
| Alternative names: | Amyloid precursor protein secretase APP secretase APPS CATB_HUMAN Cathepsin B heavy chain Cathepsin B1 CathepsinB CPSB CTSB cysteine protease OTTHUMP00000116009 OTTHUMP00000229510 OTTHUMP00000229511 OTTHUMP00000229512 OTTHUMP00000229514 OTTHUMP00000229515 OTTHUMP00000229516 Preprocathepsin B |
Images
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Fig1:
Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-Cathepsin B (bs-1500R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46/28 kD Observed band size: 30 kD |
|
Fig2:
Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin B Polyclonal Antibody, Unconjugated(bs-1500R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
|
Fig3:
Blank control: RSC96(blue). Primary Antibody:Rabbit Anti- Cathepsin B antibody(bs-1500R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Antibody (bs-1500R, 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1500R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.