| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse, Cow, Dog |
| Applications: | IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 41 kDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human CCR3: 251-355/355 |
| Positive control: | In eosinophils as well as trace amounts in neutrophils and monocytes. |
| Subcellular location: | Cell membrane; Multi-pass membrane protein. |
| Recommended Dilutions:
IHC-P IF-tissue |
1:100-500 1:100-500 |
| Uniprot #: | SwissProt : P51677 Human SwissProt : P51678 Mouse SwissProt : O54814 Rat |
| Alternative names: | B chemokine receptor b-chemokine receptor C C chemokine receptor type 3 C C CKR 3 C-C chemokine receptor type 3 C-C CKR-3 CC chemokine receptor 3 CC chemokine receptor type 3 CC CKR 3 CC R3 CC-CKR-3 CCCKR3 CCR 3 CCR-3 CCR3 CCR3_HUMAN CD 193 CD193 CD193 antigen Chemokine (C C motif) receptor 3 chemokine (C-C motif) receptor 3 Chemokine (CC) receptor 1 like 2 Chemokine CC motif receptor 3 Chemokine receptor 3 CK R3 CKR 3 CKR3 CMKB R3 CMKBR 3 CMKBR1L2 CMKBR3 Eosinophil CC chemokine receptor 3 Eosinophil eotaxin receptor Eotaxin receptor Macrophage inflammatory protein 1 alpha receptor like 2 MGC102841 MIP1 Alpha RL2 |
|
Fig1:
Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CCR3 Polyclonal Antibody, Unconjugated(bs-1167R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
|
Fig2:
Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CCR3 Polyclonal Antibody, Unconjugated(bs-1167R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |