CD45 Rabbit Polyclonal Antibody
cat.: ER1905-93
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 137 KDa
Isotype: IgG
Immunogen: KLH conjugated synthetic peptide derived from rat CD45/B220 501-600/1273
Positive control: Raji cell, Uterus (Mouse) Lysate, MCF-7 (Human) Lysate, Molt4 cell, Jurkat cell.
Subcellular location: Membrane; Single-pass type I membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts.
Recommended Dilutions:
  FC
1μg/Test
Uniprot #: SwissProt: P04157 Rat | P08575 Human
Alternative names: B220 CD 45 CD45 CD45 antigen CD45R GP180 L-CA LCA Leukocyte common antigen loc Ly-5 LY5 Ly5 homolog of Lyt-4 OTTHUMP00000033813 OTTHUMP00000033816 OTTHUMP00000033817 OTTHUMP00000038574 Protein tyrosine phosphatase receptor type c polypeptide Protein tyrosine phosphatase receptor type C protein tyrosine phosphatase receptor type C Protein tyrosine phosphatase receptor type c polypeptide Ptprc PTPRC_HUMAN Receptor-type tyrosine-protein phosphatase C T200 T200 glycoprotein T200 leukocyte common antigen
Images
ER1905-93_1.jpg Fig1: Blank control: Raji(blue). Primary Antibody:Rabbit Anti- CD45 antibody(bs-4819R), Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
ER1905-93_2.jpg Fig2: Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-CD45/PTPRC/FITC Conjugated antibody (bs-4819R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .

Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
ER1905-93_3.jpg Fig3: Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-CD45 antibody (bs-4819R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
ER1905-93_4.jpg Fig4: Blank control: Jurkat.
Primary Antibody (green line): Rabbit Anti-CD45 antibody (bs-4819R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
ER1905-93_5.jpg Fig5: Blank control:U937.
Primary Antibody (green line): Rabbit Anti-CD45 antibody (bs-4819R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.