CD80 Rabbit Polyclonal Antibody
cat.: ER1905-99
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IHC-P, IF-Cell, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 30 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from rat CD80 251-321/321 |
| Positive control: | Expressed on activated B-cells, macrophages and dendritic cells. |
| Subcellular location: | Membrane; Single-pass type I membrane protein. |
| Recommended Dilutions:
WB IHC-P IF-cell IF-tissue |
1:500-2000 1:100-500 1:100 1:100-500: 1:5000-10000 |
| Uniprot #: | SwissProt: G3V671 Rat | P33681 Human | Q00609 Mouse |
| Alternative names: | Activation B7-1 antigen B lymphocyte activation antigen B7 B7 B7-1 B7-1 antigen BB1 CD28 antigen ligand 1 CD28LG CD28LG1 CD80 CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) CD80 antigen CD80 molecule CD80_HUMAN Costimulatory factor CD80 costimulatory molecule variant IgV-CD80 CTLA-4 counter-receptor B7.1 LAB7 T-lymphocyte activation antigen CD80 CD80_RAT. |
Images
|
Fig1:
Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug Lane 3: Thymus (Rat) Lysate at 40 ug Lane 4: Raji (Human) Cell Lysate at 30 ug Lane 5: K562 (Human) Cell Lysate at 30 ug Primary: Anti-CD80 (bs-1479R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60-65 kD Observed band size: 60 kD |
|
Fig2:
Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug Lane 3: Thymus (Rat) Lysate at 40 ug Lane 4: Raji (Human) Cell Lysate at 30 ug Lane 5: K562 (Human) Cell Lysate at 30 ug Primary: Anti-CD80 (bs-2211R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60-65 kD Observed band size: 60 kD |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.