Collagen II Rabbit Polyclonal Antibody
cat.: ER1906-49
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 117 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Collagen II 231-330/1487 |
| Positive control: | Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:100-500 1:100-500 |
| Uniprot #: | SwissProt: P02458 Human |
| Alternative names: | Alpha 1 type II collagen Alpha-1 type II collagen AOM Cartilage collagen Chondrocalcin CO2A1_HUMAN COL11A3 Col2a1 Collagen 2 Collagen II alpha 1 polypeptide SEDC |
Images
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Fig1:
Sample: 293T(Human) Cell Lysate at 30 ug A549 (Human) Cell Lysate at 30 ug LOVO (Human) Cell Lysate at 40 ug MCF-7 (Human) Cell Lysate at 30 ug HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-Collagen II (bs-10589R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 117 kD |
|
Fig2:
Sample: MG63 (Human) Cell Lysate at 30 ug Primary: Anti-Collagen II (bs-10589R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 117 kD |
|
Fig3:
Sample: bone(Mouse) Lysate at 40 ug Primary: Anti-Collagen II(bs-10589R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kD Observed band size: 134 kD |
|
Fig4: Paraformaldehyde-fixed, paraffin embedded (mouse ear); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Collagen II) Polyclonal Antibody, Unconjugated (bs-10589R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
|
Fig5:
Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated(bs-10589R) 1:100, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.