| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CXCR2 aa 311-360. |
| Positive control: | MDA-MB-231 cell lysate, SK-Br-3 cell lysate, T-47D cell lysate, A549 cell lysate, HepG2 cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P25025 Human |
| Alternative names: | C-X-C chemokine receptor type 2 CD 182 CD182 CD182 antigen CDw128b Chemokine (CXC) receptor 2 CMKAR2 CXC-R2 CXCR 2 CXCR-2 CXCR2 CXCR2_HUMAN GRO/MGSA receptor High affinity interleukin-8 receptor B IL 8 receptor type 2 IL 8R B IL-8 receptor type 2 IL-8R B IL8 RB IL8 receptor type 2 IL8R B IL8R2 IL8RA Interleukin 8 Receptor B Interleukin 8 receptor, beta Interleukin 8 receptor, type 2 |
|
Fig1:
Western blot analysis of CXCR2 on different lysates with Rabbit anti-CXCR2 antibody (ER1906-87) at 1/5,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: T-47D cell lysate Lane 4: A549 cell lysate Lane 5: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 41 kDa Observed band size: 45 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1906-87) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |