Cyclin E1 Rabbit Polyclonal Antibody
cat.: ER1906-94
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 45 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from rat Cyclin E 375-411/411 |
| Positive control: | Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-tissue FC |
1:500-2000 1:100-500 1:100-500 1μg/Test |
| Uniprot #: | SwissProt: P39949 Rat |
| Alternative names: | CCNE Ccne1 CCNE1_HUMAN cyclin E variant ex5del cyclin E variant ex7del Cyclin E1 Cyclin Es Cyclin Et CyclinE G1/S specific cyclin E G1/S-specific cyclin-E1 |
Images
|
Fig1:
Sample: Lovo (Human) Cell Lysate at 30 ug Thymus (Mouse) Lysate at 40 ug U2os (Human) Cell Lysate at 30 ug K562 (Human) Cell Lysate at 30 ug Primary: Anti- Cyclin E1 (bs-0573R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kD |
|
Fig2:
Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cyclin-E Polyclonal Antibody, Unconjugated(bs-0573R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
|
Fig3:
Tissue/cell: rat testis tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cyclin E Polyclonal Antibody, Unconjugated(bs-0573R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. |
|
Fig4:
Cell: NIH/3T3 Concentration:1:100 Host/Isotype:Rabbit/IgG Flow cytometric analysis of primary antibody (Cat#: bs-0573R) on NIH/3T3(green) compared with Rabbit IgG isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody . |
|
Fig5:
Blank control (blue line): Mouse spleen cells (blue). Primary Antibody (green line): Rabbit Anti-Cyclin E1 antibody (bs-0573R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
|
Fig6:
Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Cyclin E1 antibody (bs-0573R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.