DC-SIGN Rabbit Polyclonal Antibody
cat.: ER1907-23
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IF-Tissue, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 45 KDa
Isotype: IgG
Immunogen: KLH conjugated synthetic peptide derived from human DC-SIGN/CD209 51-150/1404
Positive control: Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.
Subcellular location: Isoform 1, 2, 3, 4, 5, : Cell membrane; Single-pass type II membrane protein (Probable). Isoform 6, 7, 8, 9, 10, 11, 12: Secreted (Probable).
Recommended Dilutions:
  WB
  IHC-P
  IF-tissue
  FC
1:500-2000
1:100-500
1:100-500
1μg/Test
Uniprot #: SwissProt: Q9NNX6 Human
Alternative names: C type lectin domain family 4 member L C-type lectin domain family 4 member L CD 209 CD209 CD209 antigen CD209 antigen-like protein A CD209 molecule CD209_HUMAN Cd209a CDSIGN CIRE CLEC4L DC SIGN DC SIGN1 DC-SIGN DC-SIGN1 DCSIGN Dendritic cell specific ICAM 3 grabbing nonintegrin 1 Dendritic cell specific ICAM3 grabbing nonintegrin 1 Dendritic cell-specific ICAM-3-grabbing non-integrin 1 Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin Dengue fever protection against included Dentritic Cell Specific ICAM3 Grabbing Nonintegrin HIV GP120 Binding Protein MGC129965 MGC130443 SIGN-R1 SIGNR5
Images
ER1907-23_1.jpg Fig1: Sample:
Hela Cell Lysate at 40 ug
Hcclm3 Cell Lysate at 40 ug
Primary: Anti-DC-SIGN (bs-10053R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
ER1907-23_2.jpg Fig2: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DC-SIGN) Polyclonal Antibody, Unconjugated (bs-10053R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
ER1907-23_3.jpg Fig3: Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DC-SIGN) Polyclonal Antibody, Unconjugated (bs-10053R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
ER1907-23_4.jpg Fig4: Blank control (blue line): MCF7 (blue).
Primary Antibody (green line): Rabbit Anti-DC-SIGN antibody（bs-10053）
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC.
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.