DSPP Rabbit Polyclonal Antibody
cat.: ER1907-81
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 47/129 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Dentin sialoprotein 31-130/1301 |
| Positive control: | Expressed in teeth. DPP is synthesized by odontoblast and transiently expressed by pre-ameloblasts. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:100-500 1:100-500 |
| Uniprot #: | SwissProt: Q9NZW4 Human |
| Alternative names: | Dentin phosphophoryn Dentin phosphoprotein dentin phosphoryn Dentin sialophosphoprotein Dentin sialophosphoprotein precursor Dentin sialoprotein dentinogenesis imperfecta 1 DFNA39 DGI1 DMP3 DPP DSP Dspp DSPP_HUMAN DTDP2 |
Images
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Fig1:
Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: SW480 (Human) Cell Lysate at 30 ug Lane 3: Du145 (Human) Cell Lysate at 30 ug Primary: Anti-DSPP (bs-10316R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 140 kD Observed band size: 130 kD |
|
Fig2: Sample: LOVO(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti-DSPP (bs-10316R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:140 kD Observed band size: 130 kD |
|
Fig3:
Sample: EC-9706(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-DSPP (bs-10316R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47/129 kD Observed band size: 129 kD |
|
Fig4:
Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DSPP Polyclonal Antibody, Unconjugated(bs-10316R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.