KAT3B / p300 Rabbit Polyclonal Antibody
cat.: ER1908-18
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Green monkey
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 264 kDa
Isotype: IgG
Immunogen: Recombinant protein within human KAT3B / p300 aa 51-350.
Positive control: 293T cell lysate, HeLa cell lysate, LoVo cell lysate, C2C12 cell lysate, PC-12 cell lysate, COS-1 cell lysate.
Subcellular location: Cytoplasm, Nucleus, Chromosome.
Recommended Dilutions:
  WB

1:20,000
Uniprot #: SwissProt: Q09472 Human | B2RWS6 Mouse
Entrez Gene: 170915 Rat
Alternative names: E1A associated protein p300 E1A binding protein p300 E1A-associated protein p300 EP300 EP300: E1A binding protein p300 EP300_HUMAN Histone acetyltransferase p300 KAT3B p300 HAT RSTS2
Images
ER1908-18_1.jpg Fig1: Western blot analysis of KAT3B / p300 on different lysates with Rabbit anti-KAT3B / p300 antibody (ER1908-18) at 1/20,000 dilution.

Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: LoVo cell lysate
Lane 4: C2C12 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: COS-1 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 264 kDa
Observed band size: 300 kDa

Exposure time: 3 minutes; ECL: K1801;

3-8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1908-18) at 1/20,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.