| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 264 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human KAT3B / p300 aa 51-350. |
| Positive control: | 293T cell lysate, HeLa cell lysate, LoVo cell lysate, C2C12 cell lysate, PC-12 cell lysate, COS-1 cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus, Chromosome. |
| Recommended Dilutions:
WB |
1:20,000 |
| Uniprot #: | SwissProt: Q09472 Human | B2RWS6 Mouse Entrez Gene: 170915 Rat |
| Alternative names: | E1A associated protein p300 E1A binding protein p300 E1A-associated protein p300 EP300 EP300: E1A binding protein p300 EP300_HUMAN Histone acetyltransferase p300 KAT3B p300 HAT RSTS2 |
|
Fig1:
Western blot analysis of KAT3B / p300 on different lysates with Rabbit anti-KAT3B / p300 antibody (ER1908-18) at 1/20,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: LoVo cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 264 kDa Observed band size: 300 kDa Exposure time: 3 minutes; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1908-18) at 1/20,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |