Frizzled 7 Rabbit Polyclonal Antibody
cat.: ER1909-03
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 60 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Frizzled 7 501-574/574 |
| Positive control: | High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas. |
| Subcellular location: | Membrane; Multi-pass membrane protein. |
| Recommended Dilutions:
WB FC |
1:500-2000 3ug/Test |
| Uniprot #: | SwissProt: O75084 Human |
| Alternative names: | Frizzled 7 seven transmembrane spanning receptor frizzled class receptor 7 Frizzled drosophila homolog of 7 Frizzled family receptor 7 Frizzled homolog 7 Frizzled-7 Frizzled7 Fz 7 Fz-7 Fz7 FZD 7 FZD7 FZD7_HUMAN FzE 3 FzE3 hFz 7 hFz7 Homolog of Drosophila Frizzled 7 OTTHUMP00000163742 |
Images
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Fig1:
Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-Frizzled 7 (bs-5125R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD |
|
Fig2:
Sample: HepG2(Human) Cell Lysate at 30 ug Muscle (Mouse) Lysate at 40 ug Muscle (Rat) Lysate at 40 ug Primary: Anti-Frizzled 7 (bs-5125R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD |
|
Fig3:
Blank control: A431. Primary Antibody (green line): Rabbit Anti-Frizzled 7 antibody (bs-5125R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.