Galectin 9 Rabbit Polyclonal Antibody
cat.: ER1909-31
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Galectin 9 aa 51-100 / 355. |
| Positive control: | THP-1 cell lysate, Mouse liver tissue lysate, Mouse colon tissue lysate, Rat colon tissue lysate, mouse colon tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: O00182 Human | O08573 Mouse | P97840 Rat |
| Alternative names: | 36 kDa beta-galactoside-binding lectin Ecalectin Gal-9 galectin 9 Galectin-9 galectin9 HOM HD 21 HOMHD21 HUAT Lectin galactoside binding soluble 9 LEG9_HUMAN LGAL S9 LGALS 9 Lgals9 LGALS9A MGC117375 MGC125973 MGC125974 Tumor antigen HOM-HD-21 UAT Urate transporter/channel Urate transporter/channel protein |
Images
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Fig1:
Western blot analysis of Galectin 9 on different lysates with Rabbit anti-Galectin 9 antibody (ER1909-31) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: Mouse liver tissue lysate (40 µg/Lane) Lane 3: Mouse colon tissue lysate (40 µg/Lane) Lane 4: Rat colon tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 34 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1909-31) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Galectin 9 antibody (ER1909-31) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1909-31) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.