GDF15 Rabbit Polyclonal Antibody
cat.: ER1909-46
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Tissue
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 12.5/34 KDa
Isotype: IgG
Immunogen: KLH conjugated synthetic peptide derived from human GDF-15 221-308/308
Positive control: Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IHC-P
  IF-tissue

1:500-2000
1:100-500
1:100-500
Uniprot #: SwissProt: Q99988 Human
Alternative names: GDF 15 GDF-15 Gdf15 GDF15_HUMAN Growth differentiation factor 15 Growth/differentiation factor 15 Macrophage inhibitory cytokine 1 MIC 1 Mic-1 MIC1 NAG 1 NAG-1 NAG1 NRG 1 NRG-1 NRG1 NSAID (nonsteroidal anti inflammatory drug) activated protein 1 NSAID NSAID regulated protein 1 NSAID-activated gene 1 protein NSAID-regulated gene 1 protein PDF PLAB Placental bone morphogenetic protein Placental bone morphogenic protein Placental TGF beta Placental TGF-beta Prostate differentiation factor PTGF beta PTGFB
Images
ER1909-46_1.jpg Fig1: Sample: Lane 1: Urinary bladder (Mouse) Lysate at 40 ug Lane 2: Large intestine (Mouse) Lysate at 40 ug Lane 3: Urinary bladder (Rat) Lysate at 40 ug Lane 4: Large intestine (Rat) Lysate at 40 ug Lane 5: HT1080 (Human) cell Lysate at 40 ug Lane 6: HepG2 (Human) cell Lysate at 40 ug Lane 7: K562 (Human) cell Lysate at 40 ug Lane 8: A549 (Human) cell Lysate at 40 ug Primary: Anti-GDF15 (bs-3818R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kD
ER1909-46_2.jpg Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (GDF15) Polyclonal Antibody, Unconjugated (bs-3818R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.