| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 12.5/34 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human GDF-15 221-308/308 |
| Positive control: | Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:100-500 1:100-500 |
| Uniprot #: | SwissProt: Q99988 Human |
| Alternative names: | GDF 15 GDF-15 Gdf15 GDF15_HUMAN Growth differentiation factor 15 Growth/differentiation factor 15 Macrophage inhibitory cytokine 1 MIC 1 Mic-1 MIC1 NAG 1 NAG-1 NAG1 NRG 1 NRG-1 NRG1 NSAID (nonsteroidal anti inflammatory drug) activated protein 1 NSAID NSAID regulated protein 1 NSAID-activated gene 1 protein NSAID-regulated gene 1 protein PDF PLAB Placental bone morphogenetic protein Placental bone morphogenic protein Placental TGF beta Placental TGF-beta Prostate differentiation factor PTGF beta PTGFB |
|
Fig1: Sample: Lane 1: Urinary bladder (Mouse) Lysate at 40 ug Lane 2: Large intestine (Mouse) Lysate at 40 ug Lane 3: Urinary bladder (Rat) Lysate at 40 ug Lane 4: Large intestine (Rat) Lysate at 40 ug Lane 5: HT1080 (Human) cell Lysate at 40 ug Lane 6: HepG2 (Human) cell Lysate at 40 ug Lane 7: K562 (Human) cell Lysate at 40 ug Lane 8: A549 (Human) cell Lysate at 40 ug Primary: Anti-GDF15 (bs-3818R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kD |
|
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (GDF15) Polyclonal Antibody, Unconjugated (bs-3818R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. |