| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 61 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human GGT1 351-460/569 |
| Positive control: | Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Isoform 3 is lung-specific. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein. |
| Subcellular location: | Membrane; Single-pass type II membrane protein. |
| Recommended Dilutions:
WB |
1:500-2000 |
| Uniprot #: | SwissProt: P19440 Human |
| Alternative names: | CD224 D22S672 D22S732 Gamma glutamyl transpeptidase Gamma glutamyltransferase 1 Gamma glutamyltranspeptidase 1 Gamma-glutamyltransferase 1 Gamma-glutamyltranspeptidase 1 light chain GGT 1 GGT GGT1 GGT1_HUMAN Glutamyl transpeptidase Glutathione hydrolase 1 GTG Leukotriene C4 hydrolase MGC96892 MGC96904 MGC96963 OTTHUMP00000028921 OTTHUMP00000197959 |
|
Fig1:
Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti-GGT1 (bs-2976R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD |
|
Fig2:
Sample: Lane 1: Liver (Mouse) Tissue Lysate at 40 ug Lane 2: Liver (Rat) Tissue Lysate at 40 ug Lane 3: Thymus (Rat) Tissue Lysate at 40 ug Lane 4: A549 (Human) Cell Lysate at 30 ug Lane 5: U937 (Human) Cell Lysate at 30 ug Lane 6: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-GGT1 (bs-2976R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD |
|
Fig3:
Sample: Lane 1: Mouse kidney lysates Lane 2: Mouse Stomach lysates Lane 3: Mouse Large intestine lysates Primary: Anti-GGT1 (bs-2976R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa |