| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 55 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human PH-4 201-300/502 |
| Positive control: | Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts. |
| Subcellular location: | Endoplasmic reticulum membrane; Single-pass type II membrane protein. |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:100-500 1:100-500 |
| Uniprot #: | SwissProt: Q9NXG6 Human |
| Alternative names: | EGLN4 FLJ20262 HIF prolyl hydroxylase PH4 HIF-PH4 HIF-prolyl hydroxylase 4 HIFPH4 HPH-4 Hypoxia inducible factor prolyl 4 hydroxylase Hypoxia inducible factor prolyl hydroxylase Hypoxia-inducible factor prolyl hydroxylase 4 P4H TM P4H with transmembrane domain P4H-TM P4htm P4HTM_HUMAN PH 4 PH4 PHD4 Proline 4 hydroxylase Prolyl 4 hydroxylase transmembrane (endoplasmic reticulum) Prolyl hydroxlase domain containing 4 Transmembrane prolyl 4-hydroxylase |
|
Fig1:
Sample: Muscle (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- HIF Prolyl Hydroxylases (bs-1871R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD |
|
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF Prolyl Hydroxylases) Polyclonal Antibody, Unconjugated (bs-1871R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |