HIF Prolyl Hydroxylases Rabbit Polyclonal Antibody
cat.: ER1910-60
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Mouse, Rat
Applications: WB, IHC-P, IF-Tissue
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 55 KDa
Isotype: IgG
Immunogen: KLH conjugated synthetic peptide derived from human PH-4 201-300/502
Positive control: Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.
Subcellular location: Endoplasmic reticulum membrane; Single-pass type II membrane protein.
Recommended Dilutions:
  WB
  IHC-P
  IF-tissue

1:500-2000
1:100-500
1:100-500
Uniprot #: SwissProt: Q9NXG6 Human
Alternative names: EGLN4 FLJ20262 HIF prolyl hydroxylase PH4 HIF-PH4 HIF-prolyl hydroxylase 4 HIFPH4 HPH-4 Hypoxia inducible factor prolyl 4 hydroxylase Hypoxia inducible factor prolyl hydroxylase Hypoxia-inducible factor prolyl hydroxylase 4 P4H TM P4H with transmembrane domain P4H-TM P4htm P4HTM_HUMAN PH 4 PH4 PHD4 Proline 4 hydroxylase Prolyl 4 hydroxylase transmembrane (endoplasmic reticulum) Prolyl hydroxlase domain containing 4 Transmembrane prolyl 4-hydroxylase
Images
ER1910-60_1.jpg Fig1: Sample:
Muscle (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- HIF Prolyl Hydroxylases (bs-1871R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
ER1910-60_2.jpg Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF Prolyl Hydroxylases) Polyclonal Antibody, Unconjugated (bs-1871R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.