hnRNP A2B1 Rabbit Polyclonal Antibody
cat.: ER1910-69
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 39 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human hnRNP A2B1 101-200/353 |
| Subcellular location: | Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:100-500 1:100-500 |
| Uniprot #: | SwissProt: P22626 Human |
| Alternative names: | Heterogeneous nuclear ribonucleoprotein A2 Heterogeneous nuclear ribonucleoprotein A2/B1 Heterogeneous nuclear ribonucleoprotein B1 Heterogeneous nuclear ribonucleoproteins A2/B1 hnRNP A2 / hnRNP B1 hnRNP A2 hnRNP A2/B1 hnRNP B1 hnRNP-A2 hnRNP-B1 hnRNPA2 Hnrnpa2b1 hnRNPB1 HNRPA2 HNRPA2B1 HNRPB1 Nuclear ribonucleoprotein particle A2 protein RNP A2 RNP B1 RNP-A2 RNP-B1 RNPA2 RNPB1 ROA2_HUMAN SNRPB1 |
Images
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Fig1:
Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-hnRNP A2B1 (bs-9091R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kD |
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Fig2: Paraformaldehyde-fixed, paraffin embedded (rat intestine tissue ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (hnRNP A2B1) Polyclonal Antibody, Unconjugated (bs-9091R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (hnRNP A2B1) Polyclonal Antibody, Unconjugated (bs-9091R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (hnRNP A2B1) Polyclonal Antibody, Unconjugated (bs-9091R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.