IGFBP3 Rabbit Polyclonal Antibody
cat.: ER1911-12
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 30-35 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human IGFBP3 186-292/292 |
| Positive control: | Expressed by most tissues. Present in plasma. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P IF-tissue |
1:100-500 1:100-500 |
| Uniprot #: | SwissProt: P17936 Human |
| Alternative names: | Acid stable subunit of the 140 K IGF complex Binding protein 29 Binding protein 53 BP 53 BP53 Growth hormone dependent binding protein IBP 3 IBP-3 IBP3 IBP3_HUMAN IGF binding protein 3 IGF-binding protein 3 IGFBP 3 IGFBP-3 IGFBP3 Insulin Like Growth Factor Binding Protein 3 Insulin-like growth factor binding protein 3 precursor Insulin-like growth factor-binding protein 3 |
Images
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Fig1:
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP3 Polyclonal Antibody, Unconjugated(bs-1434R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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Fig2:
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP3 Polyclonal Antibody, Unconjugated(bs-1434R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.