Inhibin beta B Rabbit Polyclonal Antibody
cat.: ER1911-47
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 13/45 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Inhibin beta B 251-350/407 |
| Positive control: | A431 Cell Lysate, Mouse Brain Lysate, rat ovary tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:100-500 1:100-500 |
| Uniprot #: | SwissProt: P09529 Human |
| Alternative names: | Activin beta B chain Activin beta-B chain INHBB INHBB_HUMAN Inhibin beta 2 Inhibin beta B chain Inhibin beta B subunit |
Images
|
Fig1:
Sample: A431(Human) Cell Lysate at 40 ug Primary: Anti- Inhibin beta B (bs-1825R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kD |
|
Fig2:
Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti- Inhibin beta B (bs-1825R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kD |
|
Fig3:
Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Mouse Breast tissue lysates Lane 3: Mouse Placenta tissue lysates Lane 4: Mouse Liver tissue lysates Lane 5: Rat Testis tissue lysates Lane 6: Rat Breast tissue lysates Lane 7: Human A431 cell lysates Lane 8: Human HepG2 cell lysates Primary: Anti- Inhibin beta B (bs-1825R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13/45 kDa Observed band size: 46 kDa |
|
Fig4: Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Inhibin beta B) Polyclonal Antibody, Unconjugated (bs-1825R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.