Integrin Alpha V Rabbit Polyclonal Antibody
cat.: ER1911-52
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, IF-Tissue, IP |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 116 kDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Integrin Alpha V |
| Subcellular location: | Cell Membrane. |
| Recommended Dilutions:
WB IHC-P IF-cell IF-tissue IP |
1:500-2000 1:100-500 1:100 1:100-500 1:20-1:50 |
| Uniprot #: | SwissProt: P06756 Human | P43406 Mouse Entrez Gene: 296456 Rat |
| Alternative names: | antigen identified by monoclonal L230 CD 51 CD51 DKFZp686A08142 Integrin alpha five integrin alpha V beta 3 Integrin alpha-5 integrin alpha-V Integrin alpha-V light chain integrin alphaVbeta3 integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) ITAV_HUMAN ITGAV MSK 8 Msk8 Vitronectin receptor subunit alpha VNRA VTNR |
Images
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Fig1: 25 ug total protein per lane of various lysates (see on figure) probed with Integrin Alpha V polyclonal antibody, unconjugated (bs-1310R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. |
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Fig2: Paraformaldehyde-fixed, paraffin embedded Human Invasive urothelial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin Alpha V Polyclonal Antibody, Unconjugated (bs-1310R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining. |
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Fig3: Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin Alpha V Polyclonal Antibody, Unconjugated (bs-1310R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining. |
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Fig4: Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin Alpha V Polyclonal Antibody, Unconjugated (bs-1310R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.