| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 33 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Livin 101-200/298 |
| Positive control: | Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes. |
| Subcellular location: | Nucleus. Cytoplasm. Nuclear, and in a filamentous pattern throughout the cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:100-500 1:100-500 |
| Uniprot #: | SwissProt: Q96CA5 Human |
| Alternative names: | Baculoviral IAP repeat containing 7 Baculoviral IAP repeat containing protein 7 Baculoviral IAP repeat-containing protein 7 BIRC 7 birc7 BIRC7_HUMAN KIAP Kidney inhibitor of apoptosis protein Livin Livin inhibitor of apotosis Melanoma inhibitor of apoptosis protein ML IAP ML-IAP MLIAP RING finger protein 50 RNF 50 RNF50 |
|
Fig1:
Sample:Lymph node (Mouse) Lysate at 40 ug Primary: Anti-Livin (bs-0738R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 35 kD |
|
Fig2:
Tissue/cell: Human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Livin Polyclonal Antibody, Unconjugated(bs-0738R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |