Livin Rabbit Polyclonal Antibody
cat.: ER1912-40
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P, IF-Tissue
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 33 KDa
Isotype: IgG
Immunogen: KLH conjugated synthetic peptide derived from human Livin 101-200/298
Positive control: Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes.
Subcellular location: Nucleus. Cytoplasm. Nuclear, and in a filamentous pattern throughout the cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  IF-tissue

1:500-2000
1:100-500
1:100-500
Uniprot #: SwissProt: Q96CA5 Human
Alternative names: Baculoviral IAP repeat containing 7 Baculoviral IAP repeat containing protein 7 Baculoviral IAP repeat-containing protein 7 BIRC 7 birc7 BIRC7_HUMAN KIAP Kidney inhibitor of apoptosis protein Livin Livin inhibitor of apotosis Melanoma inhibitor of apoptosis protein ML IAP ML-IAP MLIAP RING finger protein 50 RNF 50 RNF50
Images
ER1912-40_1.jpg Fig1: Sample:Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-Livin (bs-0738R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 35 kD
ER1912-40_2.jpg Fig2: Tissue/cell: Human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Livin Polyclonal Antibody, Unconjugated(bs-0738R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.