Lyn Rabbit Polyclonal Antibody
cat.: ER1912-57
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 58 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Lyn 201-300/512 |
| Positive control: | Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors. |
| Subcellular location: | Cell membrane. Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:100-500 1:100-500 |
| Uniprot #: | SwissProt: P07948 Human |
| Alternative names: | AA407514 EC 2.7.10.2 FLJ26625 Hck 2 JTK 8 JTK8 Lck/Yes related novel protein tyrosine kinase LYN LYN proto oncogene Src family tyrosine kinase LYN_HUMAN ONCOGENE LYN p53Lyn p56Lyn Tyrosine protein kinase LYN Tyrosine-protein kinase Lyn V yes 1 Yamaguchi sarcoma viral related oncogene homolog Yamaguchi sarcoma viral (v yes 1) related oncogene homolog |
Images
|
Fig1:
Sample: SP2/0(Mouse) Cell Lysate at 30 ug Primary: Anti-Lyn (bs-2906R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD |
|
Fig2:
Sample: Lane 1: Mouse Lymph node tissue lysates Lane 2: Mouse Lung tissue lysates Lane 3: Mouse Placenta tissue lysates Lane 4: Rat Lymph node tissue lysates Lane 5: Rat Placenta tissue lysates Lane 6: Human Raji cell lysates Primary: Anti-Lyn (bs-2906R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kDa Observed band size: 50 kDa |
|
Fig3:
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Lyn Polyclonal Antibody, Unconjugated(bs-2906R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
|
Fig4:
Tissue/cell: human glioma tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Lyn Polyclonal Antibody, PE conjugated (bs-2906R-PE) 1:200, used at 1:200 dilution for 60 minutes at 37°C. Excitation wavelength: 488nm, 555nm; Emission wavelength: 578nm |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.