MHC Class II Rabbit Polyclonal Antibody
cat.: ER1913-01
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 27 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human HLA class II histocompatibility antigen DM beta chain 111-200/263 |
| Positive control: | Mouse lung lysates, human lung carcinoma issue. |
| Subcellular location: | Cell membrane. Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:100-500 1:100-500 |
| Uniprot #: | SwissProt: P28068 Human |
| Alternative names: | class II antigen HLA-DM beta chain class II histocompatibility antigen Class II histocompatibility antigen M beta chain D6S221E DMB HLA class II histocompatibility antigen DM beta chain HLA-DM histocompatibility type beta chain M beta chain Major histocompatibility complex class II DM beta MHC class II antigen DMB MHC class II antigen HLA DM beta chain MHC class II HLA DMB OTTHUMP00000029257 Really interesting new gene 7 protein RING 7 RING7 |
Images
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Fig1:
Protein: mouse lung lysates at 30ug; Primary: Anti-MHC Class II (bs-8481R) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 27kD Observed band size : 30kD |
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Fig2:
Sample: Lane 1: Lung (Mouse) Lysate at 40 ug Lane 2: Cerebrum (Mouse) Lysate at 40 ug Lane 3: Lymph node (Mouse) Lysate at 40 ug Lane 4: Spleen (Mouse) Lysate at 40 ug Primary: Anti-MHC Class II (bs-8481R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29/50 kD Observed band size: 50 kD |
|
Fig3:
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MHC Class II Polyclonal Antibody, Unconjugated(bs-8481R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.