| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 30 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human MMP7 151-250/269 |
| Positive control: | Mouse Uterus Lysate. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix (Probable). |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:50-200 1:50-200: 1:2000-10000 |
| Uniprot #: | SwissProt: P09237 Human |
| Alternative names: | Matrilysin Matrin Matrix Metalloproteinase 7 Matrix metalloproteinase-7 MMP 7 MMP-7 MMP7 MMP7_HUMAN MPSL1 PUMP 1 Pump 1 protease Pump-1 protease PUMP1 Uterine matrilysin Uterine metalloproteinase |
|
Fig1: 25 ug total protein per lane of various lysates (see on figure) probed with MMP7 polyclonal antibody, unconjugated (bs-0423R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. |
|
Fig2: Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP7 Polyclonal Antibody, Unconjugated (bs-0423R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. |
|
Fig3: Paraformaldehyde-fixed, paraffin embedded Human Endometrium; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP7 Polyclonal Antibody, Unconjugated (bs-0423R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. |