NKG2D Rabbit Polyclonal Antibody
cat.: ER1913-86
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 25 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human NKG2D 121-219/219 |
| Positive control: | Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs). |
| Subcellular location: | Membrane; Single-pass type II membrane protein. |
| Recommended Dilutions:
WB FC |
1:500-2000 2μg/Test |
| Uniprot #: | SwissProt: O70215 Human |
| Alternative names: | KLRK1 natural killer cell group 2D NKG2-D isoform b Klrk1 Nkrp2 NKG2-D type II integral membrane protein NKG2-D-activating NK receptor NK cell receptor D Killer cell lectin-like receptor subfamily K NK lectin-like receptor CD314 CD 314 CD314 antigen D12S2489E Killer cell lectin like receptor subfamily K member 1 Killer cell lectin-like receptor subfamily K member 1 KLR Klrk1 NKG2 D activating NK receptor NKG2 D type II integral membrane protein NKG2-D-activating NK receptor NKG2D_HUMAN NKLLR NKR P2 Nkrp2. |
Images
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Fig1:
Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- NKG2D (bs-0938R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 26 kD |
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Fig2:
Blank control (blue line): Mouse thymus cells (blue). Primary Antibody (green line): Rabbit Anti-NKG2D antibody (bs-0938R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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Fig3:
Blank control:SW480. Primary Antibody (green line): Rabbit Anti-NKG2D antibody (bs-0938R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.