ER1914-40

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Mouse
Applications:	WB, IHC-P, IF-Tissue
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

Storage buffer:

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration:

1ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

66 KDa

Isotype:

IgG

Immunogen:

KLH conjugated synthetic peptide derived from human Optineurin 341-440/577

Positive control:

Present in acqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta.

Subcellular location:

Cytoplasm > perinuclear region. Golgi apparatus.

Recommended Dilutions:
  WB
  IHC-P
  IF-tissue

1:500-2000
1:100-500
1:100-500

Uniprot #:

SwissProt: Q96CV9 Human

Alternative names:

14.7K interacting protein Ag9 C5 ALS12 E3 14.7K interacting protein E3-14.7K-interacting protein FIP 2 FIP-2 FIP2 Glaucoma 1 open angle E (adult onset) Glaucoma 1 open angle E GLC1E HIP 7 HIP-7 HIP7 Huntingtin interacting protein 7 Huntingtin interacting protein HYPL Huntingtin interacting protein L Huntingtin yeast partner L Huntingtin-interacting protein 7 Huntingtin-interacting protein L HYPL Injury inducible protein I 55 NEMO related protein NEMO-related protein NRP Optic neuropathy inducing protein Optic neuropathy-inducing protein Optineurin OPTN OPTN_HUMAN TFIIIA IntP TFIIIA-IntP Transcription factor IIIA interacting protein Transcription factor IIIA-interacting protein Tumor necrosis factor alpha inducible cellular protein containing leucine zipper domains

	Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-Optineurin(bs-13658R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66kD Observed band size: 66kD
	Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Optineurin) Polyclonal Antibody, Unconjugated (bs-13658R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
	Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Optineurin) Polyclonal Antibody, Unconjugated (bs-13658R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.